Angry staff have been filmed throwing medical gloves and shouting "go away" at the Spanish prime minister after his visit to a hospital where a nurse has ebola.
Teresa Romero, 44, caught the deadly disease while treating a missionary there. He had flown to Madrid after becoming infected in West Africa and later died.
Questions have been raised about safety measures at Carlos III hospital where Mrs Romero is in a stable but serious condition.
There have been complaints from medical staff that they did not receive the appropriate training.
Nurses' union representative Paloma Panillas said nursing staff "don't trust this situation".
Health officials revealed Mrs Romero had twice entered the missionary's room - once to change an incontinence pad and then to retrieve items after he had died.
She then accidentally touched her face while still wearing gloves used during the clean up.
Prime Minister Mariano Rajoy, who was leaving in his convoy when the medical staff shouted at him, said it was extremely unlikely the disease would spread in Spain.
Speaking outside the hospital, the leader said: "Our first priority is Teresa Romero - she is the only person that we know has the illness."
Mr Rajoy said he had set up a committee headed by the deputy prime minister to handle the crisis.
Seven more people have been admitted to the hospital in relation to Mrs Romero's case - taking the total number under observation or being treated to 14.
The new admissions included two hairdressers who had given her a beauty treatment before she was diagnosed and hospital staff who had treated her after she was admitted on Monday.
All went voluntarily to be monitored for signs of the disease, although none of the 14, including her husband, has so far tested positive for the disease except Ms Romero.
She said she did not tell doctors she had been in contact with the virus and only found out she had the disease after reading the news online.
The nurse is the first person known to have caught the disease outside of West Africa in the current outbreak, which has killed thousands of people.
Meanwhile, a British man who died in Macedonia after suffering symptoms similar to ebola has been named as Colin Jaffray, according to Sky sources.
Public Health England said it was "unlikely" the death was caused by the virus.
Prime Minister David Cameron has defended the decision to introduce enhanced screening for ebola at Heathrow and Gatwick airports and the Eurostar rail terminals, saying it was taken on "medical advice".
Questions have been raised about the checks with a Gatwick spokesman saying the airport had not been given any instructions about how the screening should be carried out.
The move has also been criticised by health experts, with one describing it as a "complete waste of time".
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